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Higher Production Of Algnate By A Mutant Of Azotobacter Vinelandii By Fermentation

by Saria Rahim | Ms. Shagufta Saeed | Dr. Muhammad | Ms. Huma Mujahid.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2012Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1521,T] (1).

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Bioconversion Of Agricultural Wastes To Polyhydroxybutyrate By Azotobacter Vinelandii

by Tehmina Aslam | Ms. Shagufta Saeed | Dr. Aftab | Ms. Huma Mujahid.

Material type: book Book; Format: print ; Literary form: drama Publisher: 2014Dissertation note: Background Polyhydroxybutyrate (PHB) is a biopolymer. It can be used as a biodegradable thermoplastic material for waste management strategies. It can be produced by various microorganisms. A bacterium, Azotobacter vinelandii accumulates PHB as intracellular granules inside their cells in response to physiological stress such as excess of carbon sources and limitation of nutrients e.g. nitrogen and phosphorus etc. During this research work PHB was produced from agricultural wastes like wheat bran and rice polishing through fermentation and by the optimization of different parameters like water substrate ratio, incubation time, volume of inoculum, pH and nitrogen concentration. Methodology A parent strain of Azotobacter vinelandii was maintained on Jerman agar plate. Fermentation media containing wheat bran and rice polishing as substrates was used to check the production of PHB for the selected bacteria. 0.5 ml of inoculum media was added into sterilized fermentation media and incubated for 24-72 hours. After that, culture media was centrifuged. Further extraction, determination and identification of PHB were carried out by using the pellet. It was found that Azotobacter vinelandii gave maximum PHB yield (192mg/100mL) at 4% of wheat bran after 48 hours of incubation and at 5% of rice polishing after 36 hours (158mg/100mL). Wheat bran gave maximum PHB production (236mg/100mL) at 1.0mL volume of inoculum and rice polishing gave maximum yield (216mg/100mL) at 2.5mL. For wheat bran optimum pH was observed to be 7 to give higher PHB yield (256mg/100mL) and for rice polishing at pH 8.0 maximum PHB was observed (236mg/100mL). From wheat bran maximum quantity of PHB was produced at 0.2% of peptone (268mg/100mL) and at 0.3% of yeast extract (256mg/100mL) while in rice polishing based media higher PHB yield was studied at 0.25% of peptone (258mg/100mL) and at 0.2% of yeast extract (250mg/100mL). In this study Azotobacter vinelandii produced higher yield of PHB using wheat bran as compared to rice polishing. Outcomes So it is concluded that PHB produced in this work can be used in various industries like pharmaceutics, food industry and also in medical fields. It will also be helpful to reduce the pollution caused by other synthetic plastics. Availability: Items available for loan: UVAS Library [Call number: 1818,T] (1).

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